When some 50 more guests said they were ill Saturday, the Ministerio de Salud closed the Hotel Barceló Tambor to new guests.
The minister, María Luisa Ávila, said she expected the closing to last for a week while the hotel seeks out the possible source of the infection.
A number of hotel guest suffered stomach problems last week, and some had to be transferred to the local clinic in Paquera. The hotel is on the east shore of the Nicoya Peninsula south of Paquera.
Tests are being done on the kitchen, the water, the pool water and other possible sources of contamination.